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Article

A double counter-selection system for the study of null alleles of essential genes in Escherichia coli.

Gene - 21 Mar 1995

Murphy C K, Stewart E J, Beckwith J

Abstract excerpt

The ability to analyze null alleles of genes can be an important means of studying both a protein's function and its interactions with other proteins involved in a particular process. However, if the gene encodes a protein that is essential to the viability of the cell, such analysis becomes complicated because a complementing copy of the gene must be present in the cell. In order to study the effects caused by...

Topics

Alleles
Escherichia coli
Gene Deletion
Genes, Bacterial
Genetic Complementation Test
Genetic Techniques
Mutation
Plasmids
Suppression, Genetic

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 7698651
DOI: 10.1016/0378-1119(94)00920-n

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7698651-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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