Article
A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery.
The EMBO journal - 15 Sept 1995
Bost S, Belin D
Abstract excerpt
The signal sequence of the murine serine protease inhibitor PAI-2 promotes alkaline phosphatase export to the E. coli periplasm. However, high level expression of this chimeric protein interferes with cell growth. Since most suppressors of this toxic phenotype map to secA and secY, growth arrest...
Topics
- ATP-Binding Cassette Transporters
- Alkaline Phosphatase
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Base Sequence
- Biological Transport
- Carrier Proteins
- Chromosome Mapping
- Escherichia coli
- Escherichia coli Proteins
- Immunoblotting
- Maltose-Binding Proteins
- Membrane Proteins
- Molecular Sequence Data
- Monosaccharide Transport Proteins
- Mutagenesis
- Periplasmic Binding Proteins