Article
Serum-sensitive mutation of Francisella novicida: association with an ABC transporter gene.
Microbiology (Reading, England) - 1 Dec 1994
Mdluli K E, Anthony L S, Baron G S, McDonald M K, Myltseva S V, Nano F E
Abstract excerpt
Francisella novicida is a facultative intracellular pathogen that can survive and grow in macrophages by preventing phagolysosomal fusion. In this study in vitro cassette mutagenesis was used to generate a library of insertion mutants of F.novicida. Two related mutants, KM14 and KM14S, initially...
Topics
- ATP-Binding Cassette Transporters
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Culture Media
- DNA, Bacterial
- Escherichia coli
- Female
- Francisella
- Genes, Bacterial
- Genetic Complementation Test