Transformation and allelic replacement in Francisella spp.

doi:10.1099/00221287-137-12-2697

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Article

Transformation and allelic replacement in Francisella spp.

Journal of general microbiology - 1 Dec 1991

Anthony L S, Gu M Z, Cowley S C, Leung W W, Nano F E

Abstract excerpt

We describe methods for transposon mutagenesis and allelic replacement in the facultative intracellular pathogen Francisella. Recombinant clones were constructed by insertion of partially cut F. tularensis or F. novicida DNA into pUC19 and then mutagenized with a mini-Tn10-Km transposon. F. novic...

Topics

Alleles
Blotting, Southern
Blotting, Western
Cloning, Molecular
Electric Stimulation
Francisella
Francisella tularensis
Mutagenesis, Insertional
Plasmids
Restriction Mapping
Transformation, Bacterial

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