Article
Efficient isolation of mutant antigen presenting cell lines by functional selection using T cell clones.
Journal of immunological methods - 9 Jun 1995
Koarada S, Kubota E, Tokushima M, Naitoh K, Miyake K, Kimoto M
Abstract excerpt
An efficient method for the isolation of mutant antigen-presenting cell (APC) lines is described. When mixtures of transfectant APC lines TA beta z (that express A beta z/A alpha d MHC class II molecules) and hypothetical variant APC lines TA beta d (that express A beta d/A alpha d class II molecules) were cultured with and selected by autoreactive A beta z/A alpha d-restricted T cell clones, the percentage of TA...
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