A novel strategy for the generation of T cell lines lacking expression of endogenous alpha- and/or beta-chain T cell receptor genes.

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Article

A novel strategy for the generation of T cell lines lacking expression of endogenous alpha- and/or beta-chain T cell receptor genes.

Journal of immunology (Baltimore, Md. : 1950) - 1 Apr 1991

Glaichenhaus N, Davis C, Bornschlegel K, Allison J P, Shastri N

Abstract excerpt

Mutant T cell lines that do not express the endogenous alpha- and/or beta-chain genes of the TCR were generated from the alpha beta TCR/CD3+ tumor cell line C6VL with a combination of classical mutagenesis methods and selection of somatic hybrid variants. This novel strategy obviated the need for repeated mutagenesis and screening of a large number of individual clones. The loss of either the alpha- or the...

Topics

Animals
Antigens, Differentiation, T-Lymphocyte
CD3 Complex
CD4 Antigens
Cell Line
Cloning, Molecular
Flow Cytometry
Gene Expression
Genetic Complementation Test
In Vitro Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mice

Muramidase

Mutation

Receptors, Antigen, T-Cell

Receptors, Antigen, T-Cell, alpha-beta

T-Lymphocytes

Transfection

Source

Open in PubMed

Identifiers

PMID: 1826016

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1826016-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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