Article
Role of ICAM-1 in antigen presentation demonstrated by ICAM-1 defective mutants.
Journal of immunology (Baltimore, Md. : 1950) - 1 Jun 1990
Dang L H, Michalek M T, Takei F, Benaceraff B, Rock K L
Abstract excerpt
We report a methodology for selecting APC with mutations that have impaired their ability to present Ag to T cells. A20 B lymphoblastoid cells were mutagenized and then repeatedly cocultured with murine T-T hybridomas in the presence of specific Ag. During these cocultures, the T-T hybridomas kil...
Topics
- Animals
- Antigen-Presenting Cells
- Antigens, Differentiation
- Cell Adhesion Molecules
- Clone Cells
- DNA
- Flow Cytometry
- Histocompatibility Antigens Class II
- In Vitro Techniques
- Intercellular Adhesion Molecule-1
- Lymphocyte Activation
- Lymphocyte Function-Associated Antigen-1
- Mice
- Mutation
- Ovalbumin
- Receptors, Leukocyte-Adhesion
- T-Lymphocytes
- Transfection