Article
Construction of a lipopolysaccharide reporter cell line and its use in identifying mutants defective in endotoxin, but not TNF-alpha, signal transduction.
Journal of immunology (Baltimore, Md. : 1950) - 15 Sept 1998
Delude R L, Yoshimura A, Ingalls R R, Golenbock D T
Abstract excerpt
Gram-negative bacterial LPS is a potent activator of inflammatory responses. The binding of LPS to CD14 initiates signal transduction; however, the molecular processes immediately following this event remain unclear. We engineered an LPS-inducible fibroblast reporter cell line to facilitate the u...
Topics
- Acute-Phase Proteins
- Animals
- Biological Transport
- Blotting, Northern
- CHO Cells
- Carrier Proteins
- Cell Culture Techniques
- Cell Fusion
- Cell Line
- Cell Separation
- Cricetinae
- Endotoxins
- Flow Cytometry
- Genes, Reporter
- Genetic Complementation Test
- Humans
- Lipopolysaccharides
- Membrane Glycoproteins