Article
An improved procedure for the in vitro expansion of human T-lymphocyte clones for mutant analysis.
Mutation research - 1 Jun 1993
Beare D M, Aldridge K E, O'Donovan M R, Cole J
Abstract excerpt
Assays detecting mutants present in human peripheral blood T-lymphocytes have been developed to monitor the population for somatic mutations. In order to investigate the nature of the mutations, colonies are further expanded in vitro by repeated lectin stimulation. To characterise fully each mutant clone, sufficient cells (approximately 10(7)) must be available for several molecular and biochemical techniques to...
Topics
- Cell Division
- Cell Separation
- Clone Cells
- Humans
- Interleukin-2
- Mutation
- Phytohemagglutinins
- Receptors, Interleukin-2
- T-Lymphocytes