Article
DNA repair by Ogt alkyltransferase influences EMS mutational specificity.
Carcinogenesis - 1 Apr 1995
Vidal A, Abril N, Pueyo C
Abstract excerpt
Forward mutations induced by ethylmethane sulfonate (EMS) in the lacI gene of Escherichia coli were recovered from bacteria proficient or deficient in the alkyltransferase encoded by the constitutive ogt gene. EMS doses of 100 or 200 mM (Ogt+) and of 50 mM (Ogt-) were selected from the corresponding dose-response curves for DNA sequence analysis. A total of 239 induced mutations affecting the N-terminal region of...
Topics
- Bacterial Proteins
- Base Sequence
- DNA Mutational Analysis
- DNA Repair
- Ethyl Methanesulfonate
- Genes, Bacterial
- Lac Operon
- Methyltransferases
- Molecular Sequence Data
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase