Article
Mutational specificity of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea in the Escherichia coli lacl gene of O6-alkylguanine-DNA alkyltransferase-proficient and -deficient strains.
Molecular carcinogenesis - 1 Dec 1995
Jurado J, Ferrezuelo F, Pueyo C
Abstract excerpt
Forward mutations induced by 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) in the lacl gene of Escherichia coli were recovered from bacteria proficient (Ogt+ Ada+) and deficient (Ogt- Ada-) in O6-alkylguanine-DNA alkyltransferase activity. A CCNU dose of 1 mM was selected for DNA sequence a...
Topics
- Antineoplastic Agents
- Base Sequence
- DNA Repair
- Escherichia coli
- Lac Operon
- Lomustine
- Methyltransferases
- Molecular Sequence Data
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase
- Repressor Proteins
- Sensitivity and Specificity