Article
A novel mutant topoisomerase II alpha present in VP-16-resistant human melanoma cell lines has a deletion of alanine 429.
Biochemistry - 20 Sept 1994
Campain J A, Gottesman M M, Pastan I
Abstract excerpt
The human melanoma cell line FEM-X was selected in multiple steps with VP-16 (etoposide) and an inhibitor of P-glycoprotein (Campain et al., 1993). The resulting clones, FVP1b and FVP3, are highly resistant to the nonintercalative epipodophyllotoxins and exhibit moderate levels of resistance to d...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Alanine
- Alleles
- Base Sequence
- Cell Line
- DNA Primers
- DNA Topoisomerases, Type II
- Drug Resistance, Multiple
- Etoposide
- HeLa Cells
- Humans
- Melanoma
- Molecular Sequence Data
- Polymerase Chain Reaction
- Restriction Mapping
- Sequence Deletion
- Tumor Cells, Cultured