Article
Acquisition of multiple copies of a mutant topoisomerase IIalpha allele by chromosome 17 aneuploidy is associated with etoposide resistance in human melanoma cell lines.
Somatic cell and molecular genetics - 1 Nov 1995
Campain J A, Slovak M L, Schoenlein P V, Popescu N C, Gottesman M M, Pastan I
Abstract excerpt
Mutants of the human melanoma cell line, FEM-X, selected in multiple steps with VP- 16 (etoposide), are cross resistant to the epipodophyllotoxins and doxorubicin. Complementary DNA's for topoisomerase IIalpha were cloned from both FEM-X and FVP3, the most resistant mutant. Deletion of nucleotide...
Topics
- Alleles
- Aneuploidy
- Antigens, Neoplasm
- Antineoplastic Agents, Phytogenic
- Base Sequence
- Chromosomes, Human, Pair 17
- DNA Primers
- DNA Topoisomerases, Type II
- DNA, Neoplasm
- DNA-Binding Proteins
- Drug Resistance
- Etoposide
- Female
- Gene Amplification