Article
High level of aphidicolin resistance with multiple mutations in mouse FM3A cell mutants.
Somatic cell and molecular genetics - 1 Sept 1990
Ito M, Matsuhashi M, Seno T, Ayusawa D
Abstract excerpt
Spontaneous mutants of mouse FM3A cells (AC1, AC2, and AC3), highly resistant to aphidicolin (3000-, 2500-, and 300-fold increase in resistance, respectively), were isolated by multistep selection. The DNA synthesizing activity in permeabilized cells of all three mutants was substantially resistant to aphidicolin, like that in intact cells. The DNA polymerase activity in nuclear extracts in AC1 and AC3, but not...
Topics
- Animals
- Aphidicolin
- DNA Polymerase II
- DNA Replication
- Diterpenes
- Drug Resistance
- Kinetics
- Mice
- Mice, Inbred C3H
- Mutation
- Nucleotides
- Tumor Cells, Cultured