Article
Transcriptional slippage during the transcription initiation process at a mutant lac promoter in vivo.
Journal of molecular biology - 5 Jun 1993
Xiong X F, Reznikoff W S
Abstract excerpt
A C.G to A.T transversion at position +10 of the lac promoter activates a nascent sigma 70-dependent promoter (the +10A promoter). The lac +10A promoter has two unusual properties; it programs a large family of transcripts with multiple 5' ends, and its sequence bears little resemblance to other sigma 70-dependent promoters. The 5' end of the +10A in vivo mRNA was determined to contain oligo(U) sequences of...
Topics
- Base Sequence
- Binding Sites
- DNA, Bacterial
- DNA-Directed RNA Polymerases
- Deoxyribonuclease I
- Escherichia coli
- Lac Operon
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- RNA, Bacterial