Heterogeneous initiation due to slippage at the bacteriophage 82 late gene promoter in vitro.

doi:10.1021/bi00499a019

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Article

Heterogeneous initiation due to slippage at the bacteriophage 82 late gene promoter in vitro.

Biochemistry - 27 Nov 1990

Guo H C, Roberts J W

Abstract excerpt

RNAs synthesized in vitro by purified Escherichia coli RNA polymerase from a bacteriophage 82 promoter are heterogeneous at the 5' end. We show that this heterogeneity results from variable addition of extra adenine residues, allowed by slippage of the initial oligonucleotide pppAAA-OH against it...

Topics

Adenosine Triphosphate
Bacteriophages
Base Sequence
Molecular Sequence Data
Mutation
Poly A
Promoter Regions, Genetic
RNA, Viral
Templates, Genetic
Transcription, Genetic
Uridine Triphosphate

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