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Article

Heterogeneous initiation due to slippage at the bacteriophage 82 late gene promoter in vitro.

Biochemistry - 27 Nov 1990

Guo H C, Roberts J W

Abstract excerpt

RNAs synthesized in vitro by purified Escherichia coli RNA polymerase from a bacteriophage 82 promoter are heterogeneous at the 5' end. We show that this heterogeneity results from variable addition of extra adenine residues, allowed by slippage of the initial oligonucleotide pppAAA-OH against its DNA template sequence TTT. Slippage backward by one base allows another A to be added, giving pppAAAA-OH, and this...

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Identifiers

PMID: 2271677
DOI: 10.1021/bi00499a019

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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