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Article

Open complex scrunching before nucleotide addition accounts for the unusual transcription start site of E. coli ribosomal RNA promoters.

Proceedings of the National Academy of Sciences of the United States of America - 29 Mar 2016

Winkelman Jared T, Chandrangsu Pete, Ross Wilma, Gourse Richard L

Abstract excerpt

Most Escherichia coli promoters initiate transcription with a purine 7 or 8 nt downstream from the -10 hexamer, but some promoters, including the ribosomal RNA promoter rrnB P1, start 9 nt from the -10 element. We identified promoter and RNA polymerase determinants of this noncanonical rrnB P1 start site using biochemical and genetic approaches including mutational analysis of the promoter, Fe(2+) cleavage assays...

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Share to DNA-Directed RNA Polymerases
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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Expression Regulation, Bacterial

Share to Nucleic Acid Conformation

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Share to Transcription, Genetic

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Identifiers

PMID: 26976590
DOI: 10.1073/pnas.1522159113
PMCID: PMC4822585

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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