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Article

An altered-specificity DNA-binding mutant of Escherichia coli sigma70 facilitates the analysis of sigma70 function in vivo.

Molecular microbiology - 1 Jun 2005

Gregory Brian D, Nickels Bryce E, Darst Seth A, Hochschild Ann

Abstract excerpt

The sigma subunit of bacterial RNA polymerase is strictly required for promoter recognition. The primary (housekeeping) sigma factor of Escherichia coli, sigma(70), is responsible for most of the gene expression in exponentially growing cells. The fact that sigma(70) is an essential protein has complicated efforts to genetically dissect the functions of sigma(70). To facilitate the analysis of sigma(70) function...

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PMID: 15882415
DOI: 10.1111/j.1365-2958.2005.04624.x

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