Article
Isolation by fluorescence-activated cell sorting of cells of a human lymphoblastoid cell strain containing mutations in the lambda immunoglobulin gene.
Environmental and molecular mutagenesis - 1 Jan 1992
McFarland R D, Vincent J L, Smith G J
Abstract excerpt
Fluorescence-activated cell sorting (FACS) was used to establish clonal populations of a human lymphoblastoid cell strain that contain spontaneously occurring and N-methyl-N'-nitro-N-nitrosoguanidine-induced mutations in the lambda immunoglobulin gene. Multiple rounds of FACS using a monoclonal antibody specific for the membrane-expressed human lambda immunoglobulin were used to enrich the population fraction of...
Topics
- Blotting, Northern
- Cell Division
- Cell Separation
- Clone Cells
- Flow Cytometry
- Fluorescent Antibody Technique
- Humans
- Immunoglobulin lambda-Chains
- Kinetics
- Lymphocytes
- Methylnitronitrosoguanidine