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Article

High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons.

15 Dec 1991

Abstract excerpt

2fTGH is a human cell line containing the selectable marker guanine phosphoribosyltransferase regulated by alpha interferon (IFN-alpha). Two IFN-alpha-unresponsive mutants were isolated previously at a low frequency (ca. 10(-8)) by selecting mutagenized 2fTGH cells in selective medium containing 6-thioguanine and IFN-alpha. By using five rounds of mutagenesis, mutants can be isolated at an appreciably higher...

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PMID: 1837150
DOI: 10.1073/pnas.88.24.11455

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