Use of a T-lymphocyte clonal assay for determining HPRT mutant frequencies in individual rats.

doi:10.1016/0165-1161(92)90018-h

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Article

Use of a T-lymphocyte clonal assay for determining HPRT mutant frequencies in individual rats.

Mutation research - 1 Jun 1992

van Dam F J, Natarajan A T, Tates A D

Abstract excerpt

Conditions for detection and isolation of HPRT- mutants in cloned rat T-lymphocytes from individual adult Lewis rats were determined. Similar to cloning of human T-cells, best results were obtained with lectin (PHA)-primed T-lymphocytes of rats. High cloning efficiencies, occasionally exceeding 5...

Topics

Animals
Cells, Cultured
Culture Media, Serum-Free
Ethylnitrosourea
Female
Hypoxanthine Phosphoribosyltransferase
Interleukin-2
Lymph Nodes
Mutagenicity Tests
Mutation
Rats
Spleen
T-Lymphocytes

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