Article
Determination of hprt mutant and mutation frequencies and the molecular characterization of human derived in vivo T-lymphocyte mutants.
Environmental and molecular mutagenesis - 1 Jan 1995
Curry J, Rowley G T, Saddi V, Beare D, Cole J, Glickman B W
Abstract excerpt
Using a T-lymphocyte clonal assay, 73 6-thioguanine resistant T-lymphocytes were isolated from two blood samples obtained 4 months apart from a 50-year-old male subject. Sixty-six of these mutants were characterized at the DNA sequence level using cDNA. One particular single base substitution was recovered a total of 23 times. The majority of T-cell receptors (TCR) of these mutants all share a common gamma-TCR...
Topics
- Base Composition
- Base Sequence
- Cloning, Molecular
- DNA Primers
- DNA, Complementary
- Exons
- Gene Expression Regulation
- Gene Frequency
- Humans
- Lymphocyte Activation
- Male