Article
Culture and propagation of Hprt mutant T-lymphocytes isolated from mouse spleen.
Environmental and molecular mutagenesis - 1 Jan 1998
Meng Q, Skopek T R, Walker D M, Hurley-Leslie S, Chen T, Zimmer D M, Walker V E
Abstract excerpt
The optimization of the mouse lymphocyte Hprt mutation assay has been impeded by the relatively poor growth potential of mouse T-cells in vitro, which leads to low cloning efficiencies (CEs) and limited expansion of Hprt mutant clones for molecular analysis of mutations occurring in control and treated mice. In this study, the addition and manipulation of concanavalin A (Con A), mouse interleukin-2 (IL-2), and a...
Topics
- Animals
- Cell Culture Techniques
- Cell Division
- Concanavalin A
- Culture Media
- Evaluation Studies as Topic
- Hypoxanthine Phosphoribosyltransferase
- Ionomycin
- Mice
- Mutation
- Phytohemagglutinins
- Spleen
- T-Lymphocytes
- Tetradecanoylphorbol Acetate