Human T-Cell Cloning by Limiting Dilution.

doi:10.1007/978-1-0716-1311-5_14

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Article

Human T-Cell Cloning by Limiting Dilution.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Maggi Laura, Capone Manuela, Mazzoni Alessio

Abstract excerpt

Human T cells represent a heterogeneous population, including cells with different phenotypical and function properties. Despite, in the last years, several technologies were developed to investigate phenotypical properties of T cells at single cell level, in vitro T cell clone 's culture remains the only way to perform functional study on T cells at single cell levels. Here, we describe the method to obtain...

Topics

Clone Cells
Coculture Techniques
Feeder Cells
Flow Cytometry
Humans
Phenotype
Research Design
Single-Cell Analysis
T-Lymphocytes
Workflow

Source

Open in PubMed

Identifiers

PMID: 33928552
DOI: 10.1007/978-1-0716-1311-5_14

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