Random mutagenesis of a plasmid-borne glycosidase gene and phenotypic selection of mutants in Escherichia coli.

doi:10.1016/0165-7992(93)90027-s

Back to search

Article

Random mutagenesis of a plasmid-borne glycosidase gene and phenotypic selection of mutants in Escherichia coli.

Mutation research - 1 Feb 1993

Lopez-Camacho C, Polaina J

Abstract excerpt

The bglA gene from Bacillus polymyxa encodes a beta-glucosidase able to hydrolyze p-nitrophenyl-beta-D-glucopyranoside (PNPG), and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), chromogenic substrates for beta-glucosidases and beta-galactosidases respectively. A plasmid carrying the...

Topics

Bacillus
Cloning, Molecular
Dose-Response Relationship, Drug
Escherichia coli
Hydroxylamine
Hydroxylamines
Mutagenesis
Phenotype
Plasmids
Protein Engineering
Transformation, Genetic
beta-Glucosidase

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.