Article
[Molecular evolution of beta-glucuronidase in vitro: obtaining thermotolerant GUS gene].
Yi chuan xue bao = Acta genetica Sinica - 1 Jan 2002
Xiong Ai-Sheng, Yao Quan-Hong, Peng Ri-He, Chen Jian-Min, Li Xian, Fan Hui-Qin
Abstract excerpt
The Escherichia coli beta-glucuronidase gene (gus) has been developed as a reporter gene for plants, and has been widely used for over a decade. Both chromogenic and fluorogenic GUS substrates have been synthesized, allowing rapid nonradioactive assays. The use of the Escherichia coli enzyme beta-glucuronidase (GUS) as a reporter in gene expression studies is limited by some plants and plant-associated bacteria...
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