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Article

Identification of a Bacillus subtilis secretion mutant using a beta-galactosidase screening procedure.

Microbiology (Reading, England) - 1 Jul 1995

Jacobs M F, Andersen J B, Borchert T V, Kontinen V P

Abstract excerpt

High-level synthesis of exportable beta-galactosidase (LacZ) fusion proteins in Bacillus subtilis results in a lethal phenotype, and has been suggested as a tool for the selection of secretion mutants. We tested a plasmid-based, inducible lacZ fusion gene system for this purpose, but frequent mutations in cis, which reduced expression of the fusion gene, forced abandonment of the induction-selection strategy....

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PMID: 7551041
DOI: 10.1099/13500872-141-7-1771

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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