Article
Identification of a Bacillus subtilis secretion mutant using a beta-galactosidase screening procedure.
Microbiology (Reading, England) - 1 Jul 1995
Jacobs M F, Andersen J B, Borchert T V, Kontinen V P
Abstract excerpt
High-level synthesis of exportable beta-galactosidase (LacZ) fusion proteins in Bacillus subtilis results in a lethal phenotype, and has been suggested as a tool for the selection of secretion mutants. We tested a plasmid-based, inducible lacZ fusion gene system for this purpose, but frequent mut...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Biological Transport
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Genetic Vectors
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Recombinant Fusion Proteins
- Restriction Mapping
- Subtilisins
- Temperature
- Time Factors
- Transcription Factors
- Xylose
- beta-Galactosidase