Article
Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene.
Journal of general microbiology - 1 Nov 1990
Kuroda A, Sekiguchi J
Abstract excerpt
We have cloned DNA fragments from Bacillus subtilis 168S into Escherichia coli, which produced a lytic zone on an agar medium containing B. subtilis cell wall. Sequencing of the fragments showed the presence of an open reading frame (ORF) which encodes a polypeptide of 272 amino acids with a mole...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- Cell Wall
- Chromosome Mapping
- Cloning, Molecular
- Codon
- DNA, Bacterial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genetic Markers
- Hydrolases
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Restriction Mapping