Article
Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene.
Journal of general microbiology - 1 Nov 1990
Kuroda A, Sekiguchi J
Abstract excerpt
We have cloned DNA fragments from Bacillus subtilis 168S into Escherichia coli, which produced a lytic zone on an agar medium containing B. subtilis cell wall. Sequencing of the fragments showed the presence of an open reading frame (ORF) which encodes a polypeptide of 272 amino acids with a molecular mass of 29919 Da. The deduced amino acid sequence showed considerable homology with that of the cell wall...
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