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Directed evolution of epoxide hydrolase from A. radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling.

Chemistry & biology - 1 Jul 2004

van Loo Bert, Spelberg Jeffrey H Lutje, Kingma Jaap, Sonke Theo, Wubbolts Marcel G, Janssen Dick B

Abstract excerpt

The enantioselectivity of epoxide hydrolase from Agrobacterium radiobacter (EchA) was improved using error-prone PCR and DNA shuffling. An agar plate assay was used to screen the mutant libraries for activity. Screening for improved enantioselectivity was subsequently done by spectrophotometric progress curve analysis of the conversion of para-nitrophenyl glycidyl ether (pNPGE). Kinetic resolutions showed that...

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Identifiers

PMID: 15271356
DOI: 10.1016/j.chembiol.2004.04.019

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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