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Article

Determination of the roles of Glu-461 in beta-galactosidase (Escherichia coli) using site-specific mutagenesis.

The Journal of biological chemistry - 5 Apr 1990

Cupples C G, Miller J H, Huber R E

Abstract excerpt

Site-directed substitutions (Asp, Gly, Gln, His, and Lys) were made for Glu-461 of beta-galactosidase (Escherichia coli). All substitutions resulted in loss of most activity. Substrates and a substrate analog inhibitor were bound better by the Asp-substituted enzyme than by the normal enzyme, about the same for enzyme substituted with Gly, but only poorly when Gln, His, or Lys was substituted. This shows that...

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PMID: 1969405

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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