Article
Bacillus subtilis mutant deficient in the major autolytic amidase and glucosaminidase is impaired in motility.
FEMS microbiology letters - 1 Sept 1993
Rashid M H, Kuroda A, Sekiguchi J
Abstract excerpt
The purified autolytic endo-beta-N-acetylglucosaminidase of Bacillus subtilis AC327 was cleaved with cyanogen bromide, and the N-terminal amino acid sequence of one of the peptide fragments was determined. Then, a DNA fragment containing a part of the glucosaminidase gene was cloned into Escherichia coli JM109 using synthetic oligonucleotides as probes whose sequences had been deduced from the N-terminal amino...
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