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Article

Directed evolution of beta -glucosidase A from Paenibacillus polymyxa to thermal resistance.

The Journal of biological chemistry - 5 May 2000

Gonzalez-Blasco G, Sanz-Aparicio J, Gonzalez B, Hermoso J A, Polaina J

Abstract excerpt

The beta-glucosidase encoded by the bglA gene from Paenibacillus polymyxa has a half-life time of 15 min at 35 degrees C and no detectable activity at 55 degrees C. We have isolated random mutations that enhance the thermoresistance of the enzyme. Following a directed evolution strategy, we have combined some of the isolated mutations to obtain a beta-glucosidase with a half-life of 12 min at 65 degrees C, in the...

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Identifiers

PMID: 10788490
DOI: 10.1074/jbc.275.18.13708

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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