Article
Identification of catalytic residues in the beta-glucoside permease of Escherichia coli by site-specific mutagenesis and demonstration of interdomain cross-reactivity between the beta-glucoside and glucose systems.
The Journal of biological chemistry - 15 Aug 1990
Schnetz K, Sutrina S L, Saier M H, Rak B
Abstract excerpt
beta-Glucoside Enzyme II (IIBgl) of the Escherichia coli phosphotransferase system transports and phosphorylates beta-glucosides, whereas the glucose Enzyme II-III pair (IIGlc-IIIGlc) transports and phosphorylates glucose as well as certain aliphatic alpha- and beta-glucosides. Comparisons of their respective amino acid sequences previously revealed that both systems are homologous and must be evolutionarily...
Topics
- Arbutin
- Binding Sites
- Escherichia coli
- Fermentation
- Genetic Complementation Test
- Glucose
- Glucosides
- Glycosides
- Kinetics
- Mutation
- Phosphoenolpyruvate Sugar Phosphotransferase System