Article
Boundaries of the pSC101 minimal replicon are conditional.
Journal of bacteriology - 1 Sept 1995
Miller C A, Ingmer H, Cohen S N
Abstract excerpt
The DNA segment essential for plasmid replication commonly is referred to as the core or minimal replicon. We report here that host and plasmid genes and sites external to the core replicon of plasmid pSC101 determine the boundaries and competence of the replicon and also the efficiency of partitioning. Missense mutations in the plasmid-encoded RepA protein or mutation of the Escherichia coli topoisomerase I gene...
Topics
- Bacterial Proteins
- DNA Helicases
- DNA Replication
- DNA Topoisomerases, Type I
- DNA-Binding Proteins
- Escherichia coli
- Integration Host Factors
- Mutation
- Plasmids
- Proteins
- Regulatory Sequences, Nucleic Acid