Article
Isolation of the replication and partitioning regions of the Salmonella typhimurium virulence plasmid and stabilization of heterologous replicons.
Journal of bacteriology - 1 Sept 1990
Tinge S A, Curtiss R
Abstract excerpt
Although the virulence plasmid of Salmonella typhimurium has a copy number of one to two per chromosome, plasmid-free segregants are produced at a rate less than 10(-7) per cell per generation. Three regions appear to be involved in the maintenance of this virulence plasmid. The first two, repB a...
Topics
- Cloning, Molecular
- DNA Replication
- DNA, Bacterial
- Escherichia coli
- Gene Library
- Genotype
- Phenotype
- Plasmids
- Replicon
- Restriction Mapping
- Salmonella typhimurium
- Virulence
