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Article

Excess intracellular concentration of the pSC101 RepA protein interferes with both plasmid DNA replication and partitioning.

Journal of bacteriology - 1 Dec 1993

Ingmer H, Cohen S N

Abstract excerpt

RepA, a plasmid-encoded gene product required for pSC101 replication in Escherichia coli, is shown here to inhibit the replication of pSC101 in vivo when overproduced 4- to 20-fold in trans. Unlike plasmids whose replication is prevented by mutations in the repA gene, plasmids prevented from replicating by overproduction of the RepA protein were lost rapidly from the cell population instead of being partitioned...

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Identifiers

PMID: 8253672
DOI: 10.1128/jb.175.24.7834-7841.1993
PMCID: PMC206959

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Excess intracellular concentration of the pSC101 RepA protein interferes with both plasmid DNA replication and partitioning.PMID 8253672Read ↗

Isolation and characterization of plasmid mutations that enable partitioning of pSC101 replicons lacking the partition (par) locus.Semantic proximity Read ↗

The RepA protein of plasmid pSC101 controls Escherichia coli cell division through the SOS response.Semantic proximity Read ↗

Boundaries of the pSC101 minimal replicon are conditional.Semantic proximity Read ↗

In vivo and in vitro studies of a copy number mutation of the RepA replication protein of plasmid pSC101.Semantic proximity Read ↗

Role of DNA superhelicity in partitioning of the pSC101 plasmid.Semantic proximity Read ↗

Location of a P1 plasmid replication inhibitor determinant within the initiator gene.Semantic proximity Read ↗

Replication origin mutations affecting binding of pSC101 plasmid-encoded Rep initiator protein.Semantic proximity Read ↗

Identification of a megaplasmid centromere reveals genetic structural diversity within the repABC family of basic replicons.Semantic proximity Read ↗

RepA and RepB exert plasmid incompatibility repressing the transcription of the repABC operon.Semantic proximity Read ↗

Isolation and characterization of novel mutations in the pSC101 origin that increase copy number.Semantic proximity Read ↗

Rep provides a second motor at the replisome to promote duplication of protein-bound DNA.Semantic proximity Read ↗

Mutations in the E. coli Rep helicase increase the amount of DNA per cell.Semantic proximity Read ↗

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