Article
Excess intracellular concentration of the pSC101 RepA protein interferes with both plasmid DNA replication and partitioning.
Journal of bacteriology - 1 Dec 1993
Ingmer H, Cohen S N
Abstract excerpt
RepA, a plasmid-encoded gene product required for pSC101 replication in Escherichia coli, is shown here to inhibit the replication of pSC101 in vivo when overproduced 4- to 20-fold in trans. Unlike plasmids whose replication is prevented by mutations in the repA gene, plasmids prevented from repl...
Topics
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA Helicases
- DNA Primers
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Genes, Bacterial
- Genotype
- Kinetics
- Molecular Sequence Data
- Plasmids
- Polymerase Chain Reaction
- Proteins
- Restriction Mapping
- Temperature
- Trans-Activators