Article
Role of DNA superhelicity in partitioning of the pSC101 plasmid.
Cell - 13 Jul 1990
Miller C A, Beaucage S L, Cohen S N
Abstract excerpt
Previous work has shown that a cis-acting locus (termed par for partitioning) on the pSC101 plasmid accomplishes its stable inheritance in dividing cell populations. We report here that the DNA of pSC101 derivatives lacking the par region shows a decrease in overall superhelical density as compared with DNA of wild-type pSC101. Chemicals and bacterial mutations that reduce negative DNA supercoiling increase the...
Topics
- DNA Topoisomerases, Type I
- DNA, Superhelical
- Escherichia coli
- Mutation
- Phenotype
- Plasmids
- Species Specificity