Article
Molecular analysis of RepHI1A, a minimal replicon of the IncHI1 plasmid R27.
Molecular microbiology - 1 Feb 1994
Newnham P J, Taylor D E
Abstract excerpt
R27, a large conjugative plasmid of the HI incompatibility group, was subjected to a subcloning analysis which revealed the presence of a Poll-independent replicon and determinants contributing to incompatibility within a 2.7 kb SalI/XbaI fragment. The DNA sequence of the minimal replicon revealed the presence of a large open reading frame (ORF) as well two sets of 19 bp repeated oligonucleotides (iterons), in...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Molecular Sequence Data
- Open Reading Frames
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- Replicon