Article
A pSC101-par sequence-mediated study on the intracellular state of supercoiling of the pBR322 genome in Escherichia coli DNA topoisomerase I deletion mutant.
FEMS microbiology letters - 1 Jun 1992
Ishii S, Murakami T, Shishido K
Abstract excerpt
In Escherichia coli DNA topoisomerase I deletion mutant DM800, transcription of the tetracycline-resistance gene (tet) in the pBR322 genome is thought to create and maintain two domains of positive supercoils ahead, and negative supercoils behind, the transcription complex. To assess the actual intracellular state of twin-supercoiled domains, par sequence (365 bp) of plasmid pSC101, which shows a high affinity...
Topics
- Base Sequence
- Chromosome Deletion
- Chromosomes, Bacterial
- DNA Topoisomerases, Type I
- DNA, Bacterial
- DNA, Superhelical
- Escherichia coli
- Genome, Bacterial
- Mutation
- Plasmids
- Tetracycline Resistance