Article
Gyrase-dependent stabilization of pSC101 plasmid inheritance by transcriptionally active promoters.
The EMBO journal - 1 Sept 1991
Beaucage S L, Miller C A, Cohen S N
Abstract excerpt
The pSC101 plasmid encodes a cis-acting genetic locus termed par that ensures the stable inheritance of plasmids in a population of dividing cells. In the absence of selection, par-defective plasmids are lost rapidly from the bacterial population. We report here that the stability of par-deleted...
Topics
- DNA Topoisomerases, Type II
- DNA, Superhelical
- Electrophoresis, Agar Gel
- Escherichia coli
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- Restriction Mapping
- Transcription, Genetic