Article
Proofreading-defective DNA polymerase II increases adaptive mutation in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 1995
Foster P L, Gudmundsson G, Trimarchi J M, Cai H, Goodman M F
Abstract excerpt
The role of Escherichia coli DNA polymerase (Pol) II in producing or avoiding mutations was investigated by replacing the chromosomal Pol II gene (polB+) by a gene encoding an exonuclease-deficient mutant Pol II (polBex1). The polBex1 allele increased adaptive mutations on an episome in nondividing cells under lactose selection. The presence of a Pol III antimutator allele (dnaE915) reduced adaptive mutations in...
Topics
- Acclimatization
- Alleles
- Base Sequence
- Chromosomes, Bacterial
- DNA Polymerase II
- DNA Polymerase III
- DNA Primers
- Escherichia coli
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Genes, Bacterial