Article
Mutants in the Exo I motif of Escherichia coli dnaQ: defective proofreading and inviability due to error catastrophe.
Proceedings of the National Academy of Sciences of the United States of America - 2 Apr 1996
Fijalkowska I J, Schaaper R M
Abstract excerpt
The Escherichia coli dnaQ gene encodes the proofreading 3' exonuclease (epsilon subunit) of DNA polymerase III holoenzyme and is a critical determinant of chromosomal replication fidelity. We constructed by site-specific mutagenesis a mutant, dnaQ926, by changing two conserved amino acid residues...
Topics
- Amino Acid Sequence
- Chromosomes, Bacterial
- DNA Polymerase III
- DNA Replication
- Diploidy
- Escherichia coli
- Escherichia coli Proteins
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Genes, Bacterial
- Genotype
- Haploidy
- Humans
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Restriction Mapping
- Saccharomyces cerevisiae