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Article

Mutants in the Exo I motif of Escherichia coli dnaQ: defective proofreading and inviability due to error catastrophe.

Proceedings of the National Academy of Sciences of the United States of America - 2 Apr 1996

Fijalkowska I J, Schaaper R M

Abstract excerpt

The Escherichia coli dnaQ gene encodes the proofreading 3' exonuclease (epsilon subunit) of DNA polymerase III holoenzyme and is a critical determinant of chromosomal replication fidelity. We constructed by site-specific mutagenesis a mutant, dnaQ926, by changing two conserved amino acid residues (Asp-12-->Ala and Glu-14-->Ala) in the Exo I motif, which, by analogy to other proofreading exonucleases, is essential...

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Identifiers

PMID: 8610131
DOI: 10.1073/pnas.93.7.2856
PMCID: PMC39723

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