Functional linkage between the active site of alpha-lytic protease and distant regions of structure: scanning alanine mutagenesis of a surface loop affects activity and substrate specificity.

Abstract excerpt

Previous structural and kinetic characterization of mutations within the active site of alpha-lytic protease have demonstrated that amino acid residues in direct contact with the substrate are major substrate specificity determinants. The experiments described here identify residues 216-226 of alpha-lytic protease as a region of structure peripheral to the active site that also plays an important role in...

Topics

• Alanine
• Aspartic Acid Endopeptidases
• Binding Sites
• Chymotrypsin
• Endopeptidases
• Kinetics
• Molecular Structure
• Mutagenesis
• Mutation
• Pancreatic Elastase
• Protein Conformation