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Article

Mutation in the Pro-Peptide Region of a Cysteine Protease Leads to Altered Activity and Specificity-A Structural and Biochemical Approach.

PloS one - 1 Jan 2016

Dutta Sruti, Choudhury Debi, Roy Sumana, Dattagupta Jiban Kanti, Biswas Sampa

Abstract excerpt

Papain-like proteases contain an N-terminal pro-peptide in their zymogen form that is important for correct folding and spatio-temporal regulation of the proteolytic activity of these proteases. Catalytic removal of the pro-peptide is required for the protease to become active. In this study, we have generated three different mutants of papain (I86F, I86L and I86A) by replacing the residue I86 in its pro-peptide...

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Source

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Identifiers

PMID: 27352302
DOI: 10.1371/journal.pone.0158024
PMCID: PMC4924875

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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