Article
Impaired in vitro kinetics of EF-Tu mutant Aa.
European journal of biochemistry - 10 Mar 1990
Tapio S, Bilgin N, Ehrenberg M
Abstract excerpt
The kirromycin-resistant EF-Tu mutant Aa, previously shown to be an antisuppressor for nonsense and missense suppressor tRNAs, has been characterised in a poly(U)-primed translation system in vitro. Two major defects were found in the function of the mutant. First, the dissociation constant for A...
Topics
- Bacterial Proteins
- Binding Sites
- Drug Resistance
- Escherichia coli
- Guanosine Diphosphate
- Guanosine Triphosphate
- Kinetics
- Peptide Biosynthesis
- Peptide Elongation Factor Tu
- Peptide Elongation Factors
- Phenotype
- Protein Biosynthesis
- Pyridones
- RNA, Transfer
- RNA, Transfer, Amino Acid-Specific
- Ribosomal Proteins
- Suppression, Genetic