Article
The interaction between aminoacyl-tRNA and the mutant elongation factors Tu AR and B0.
Biochimica et biophysica acta - 27 Aug 1990
Abrahams J P, Acampo J J, Ott G, Sprinzl M, de Graaf J M, Talens A, Kraal B
Abstract excerpt
The binding of Tyr-[AEDANS-s2C]tRNA(Tyr) (Tyr-tRNA(Tyr) modified at the penultimate cytidine residue with a thio group at position 2 of the pyrimidine ring, to which an N-(acetylaminoethyl)-5-naphthylamine-1-sulfonic acid fluorescence group is attached) to mutant elongation factor (EF)-Tu species from E. coli, EF-TuAR (Ala-375----Thr) and EF-TuBO (Gly-222----Asp), both complexed to GTP, was investigated in...
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