Increased sensitivity to DNA-alkylating agents in CHO mutants with decreased poly(ADP-ribose) polymerase activity.

doi:10.1016/0921-8777(94)90069-8

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Increased sensitivity to DNA-alkylating agents in CHO mutants with decreased poly(ADP-ribose) polymerase activity.

Mutation research - 1 May 1994

Witmer M V, Aboul-Ela N, Jacobson M K, Stamato T D

Abstract excerpt

Using a replica-plating procedure and a 32P-NAD+ permeable cell-screening assay, we have isolated a CHO mutant, PADR-9, which displays approximately 17% of the wild-type level of poly(ADP-ribose) polymerase activity. Biochemical analysis of the mutant using activity, Western, and Northern blot te...

Topics

Alkylating Agents
Animals
CHO Cells
Cell Survival
Cricetinae
DNA Damage
Ethyl Methanesulfonate
Glycoside Hydrolases
Kinetics
Methyl Methanesulfonate
Mutagenesis
Mutagens

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

NAD

Nuclear Proteins

Poly(ADP-ribose) Polymerases

RNA

Sulfuric Acid Esters

Source

Open in PubMed

Identifiers

PMID: 7513057
DOI: 10.1016/0921-8777(94)90069-8

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7513057-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Increased sensitivity to DNA-alkylating agents in CHO mutants with decreased poly(ADP-ribose) polymerase activity.PMID 7513057Read ↗

Isolation of Chinese hamster ovary cells with reduced poly(ADP-ribose) polymerase activity.Semantic proximity Read ↗

Isolation of a human cDNA encoding amidophosphoribosyltransferase and functional complementation of a CHO Ade-A mutant deficient in this activity.Semantic proximity Read ↗

Poly(ADP ribose) polymerase-defective mutant cell clone of mouse L1210 cells.Semantic proximity Read ↗

Isolation and partial characterisation of a mammalian cell mutant hypersensitive to topoisomerase II inhibitors and X-rays.Semantic proximity Read ↗

NAD(+) consumption in carcinogen-treated hamster cells overexpressing a dominant negative mutant of poly(ADP-ribose) polymerase.Semantic proximity Read ↗

Molecular and biochemical elucidation of a cellular phenotype characterized by adenine analogue resistance in the presence of high levels of adenine phosphoribosyltransferase activity.Semantic proximity Read ↗

Aphidicolin-resistant Chinese hamster ovary cells possess altered DNA polymerases of the alpha-family.Semantic proximity Read ↗

trans-dominant inhibition of poly(ADP-ribosyl)ation sensitizes cells against gamma-irradiation and N-methyl-N'-nitro-N-nitrosoguanidine but does not limit DNA replication of a polyomavirus replicon.Semantic proximity Read ↗

Poly(ADP-ribose) polymerase null mouse cells synthesize ADP-ribose polymers.Semantic proximity Read ↗

Depletion of poly(ADP-ribose) polymerase by antisense RNA expression results in a delay in DNA strand break rejoining.Semantic proximity Read ↗

Temperature-sensitive DNA mutant of Chinese hamster ovary cells with a thermolabile ribonucleotide reductase activity.Semantic proximity Read ↗

Characterization of a Chinese hamster ovary cell mutant having a mutation in elongation factor-2.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.