Article
Poly(ADP ribose) polymerase-defective mutant cell clone of mouse L1210 cells.
Experimental cell research - 1 May 1992
Yoshihara K, Itaya A, Hironaka T, Sakuramoto S, Tanaka Y, Tsuyuki M, Inada Y, Kamiya T, Ohnishi K, Honma M
Abstract excerpt
By a sequential mutation and selection utilizing N-methyl-N'-nitro-N-nitrosoguanidine as a mutagen, we succeeded in separating a poly(ADP ribose) polymerase-defective mutant clone (Cl-3527) from a mouse L1210 cell clone (Cl-3). The enzyme activity per cell in Cl-3527 cells was only 8% of that in wild type L1210 (CCL 219) cells. Immunoblot analysis of the enzyme protein in crude extracts of the mutant and wild...
Topics
- Animals
- Base Sequence
- Cell Line
- DNA Fingerprinting
- DNA Probes
- Genetic Vectors
- Humans
- Leukemia L1210
- Mice
- Molecular Sequence Data
- Mutation
- Poly(ADP-ribose) Polymerases