Article
trans-dominant inhibition of poly(ADP-ribosyl)ation sensitizes cells against gamma-irradiation and N-methyl-N'-nitro-N-nitrosoguanidine but does not limit DNA replication of a polyomavirus replicon.
Molecular and cellular biology - 1 Jun 1995
Küpper J H, Müller M, Jacobson M K, Tatsumi-Miyajima J, Coyle D L, Jacobson E L, Bürkle A
Abstract excerpt
Poly(ADP-ribosyl)ation is a posttranslational modification of nuclear proteins catalyzed by poly(ADP-ribose) polymerase (PARP; EC 2.4.2.30), with NAD+ serving as the substrate. PARP is strongly activated upon recognition of DNA strand breaks by its DNA-binding domain. Experiments with low-molecular-weight inhibitors of PARP have led to the view that PARP activity plays a role in DNA repair and possibly also in...
Topics
- Animals
- Base Sequence
- Binding Sites
- Cell Division
- Cell Line, Transformed
- Cricetinae
- Cricetulus
- DNA-Binding Proteins
- Gamma Rays
- Gene Transfer Techniques