Article
Isolation of a human cDNA encoding amidophosphoribosyltransferase and functional complementation of a CHO Ade-A mutant deficient in this activity.
Somatic cell and molecular genetics - 1 May 1991
Barton J W, Bleskan J, Patterson D
Abstract excerpt
We report here the isolation of a human cDNA encoding the first step in de novo purine biosynthesis, amidophosphoribosyltransferase (PRAT). The human PRAT cDNA was isolated by complementation of a Saccharomyces cerevisiae ade4 mutant deficient in PRAT enzymatic activity. The identity of the isola...
Topics
- Amidophosphoribosyltransferase
- Animals
- Cricetinae
- Cricetulus
- DNA
- Genetic Complementation Test
- Genetic Markers
- Glycine
- Humans
- Hypoxanthine
- Hypoxanthines
- Liver Neoplasms, Experimental
- Mutation
- RNA, Messenger
- Ribonucleotides
- Saccharomyces cerevisiae
- Tumor Cells, Cultured